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CoraLite®594-conjugated NEK2 Monoclonal antibody

NEK2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D7B4

Cat no : CL594-66632

Print datasheet

Synonyms

Never in mitosis A-related kinase 2, NEK2A, HsPK21, HSPK 21, EC:2.7.11.1

验证数据展示

  • FC experiment of HeLa using CL594-66632

    FC experiment of HeLa using CL594-66632

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human NEK2 (CL594-66632, Clone:3D7B4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NEK2 Ready-To-Use IHC Kit. NEK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66632-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66632 targets NEK2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen NEK2 fusion protein Ag21476 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NIMA (never in mitosis gene a)-related kinase 2
Synonyms Never in mitosis A-related kinase 2, NEK2A, HsPK21, HSPK 21, EC:2.7.11.1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC043502
Gene Symbol NEK2
Gene ID (NCBI) 4751
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP51955
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEK2, also named as HSPK 21, belongs to the NEK family. NEK2 is well recognized as a multifunctional protein with roles in cell cycle regulation, such as centrosome duplication and separation, microtubule stabilization, kinetochore attachment and spindle assembly checkpoint. NEK2 is highly expressed in several cancer types, such as cholangiocarcinoma, breast, colorectal, and pancreatic cancer. NEK2 in mammals has three splice variants: NEK2A, NEK2B, and NEK2C. NEK2A is evenly distributed within the nuclei and cytoplasm, while NEK2B is mainly distributed in the cytoplasm, and NEK2C is mainly distributed in the nuclear region. As NEK2A, NEK2B and NEK2C exhibit overlapping or identical substrate usage, these variants are collectively referred to here as NEK2 (PMID:31092416).

实验方案

Product Specific Protocols
FC protocol for CL594 NEK2 antibody CL594-66632Download protocol
Standard Protocols
Click here to view our Standard Protocols