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验证数据展示

  • FC experiment of HeLa using CL594-66632

    FC experiment of HeLa using CL594-66632

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human NEK2 (CL594-66632, Clone:3D7B4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated NEK2 Monoclonal antibody

NEK2 Monoclonal Antibody for FC (Intra)
Cat No. CL594-66632

产品说明书

CloneNo. 3D7B4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

Never in mitosis A-related kinase 2, NEK2A, HsPK21, HSPK 21, EC:2.7.11.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NEK2 Ready-To-Use IHC Kit. NEK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66632-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66632 targets NEK2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 NEK2 fusion protein Ag21476 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NIMA (never in mitosis gene a)-related kinase 2
别名 Never in mitosis A-related kinase 2, NEK2A, HsPK21, HSPK 21, EC:2.7.11.1
计算分子量 52 kDa
观测分子量 48 kDa
GenBank蛋白编号BC043502
基因名称 NEK2
Gene ID (NCBI) 4751
RRIDAB_2923922
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP51955
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEK2, also named as HSPK 21, belongs to the NEK family. NEK2 is well recognized as a multifunctional protein with roles in cell cycle regulation, such as centrosome duplication and separation, microtubule stabilization, kinetochore attachment and spindle assembly checkpoint. NEK2 is highly expressed in several cancer types, such as cholangiocarcinoma, breast, colorectal, and pancreatic cancer. NEK2 in mammals has three splice variants: NEK2A, NEK2B, and NEK2C. NEK2A is evenly distributed within the nuclei and cytoplasm, while NEK2B is mainly distributed in the cytoplasm, and NEK2C is mainly distributed in the nuclear region. As NEK2A, NEK2B and NEK2C exhibit overlapping or identical substrate usage, these variants are collectively referred to here as NEK2 (PMID:31092416).

实验方案

Product Specific Protocols
FC protocol for CL594 NEK2 antibody CL594-66632Download protocol
Standard Protocols
Click here to view our Standard Protocols