TNFAIP3 Monoclonal antibody

TNFAIP3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3A11G6

Cat no : 66695-1-Ig

Print datasheet

Synonyms

OTUD7C, OTU domain-containing protein 7C, EC:2.3.2.-, A20p50, A20p37



经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inmouse kidney tissue, human thyroid cancer tissue, rat ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TNFAIP3 Ready-To-Use IHC Kit. TNFAIP3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66695-1-Ig targets TNFAIP3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen TNFAIP3 fusion protein Ag18150 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor, alpha-induced protein 3
Synonyms OTUD7C, OTU domain-containing protein 7C, EC:2.3.2.-, A20p50, A20p37
Calculated Molecular Weight 790 aa, 90 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC114480
Gene Symbol TNFAIP3
Gene ID (NCBI) 7128
RRIDAB_2882048
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21580
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity.

实验方案

Product Specific Protocols
WB protocol for TNFAIP3 antibody 66695-1-IgDownload protocol
IHC protocol for TNFAIP3 antibody 66695-1-IgDownload protocol
IF protocol for TNFAIP3 antibody 66695-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Biology (Basel)

Interleukin-10 Deficiency Impacts on TNF-Induced NFκB Regulated Responses In Vivo

Authors - Stamatia Papoutsopoulou
humanWB,IHC,IF

Cell Death Dis

A20 targets PFKL and glycolysis to inhibit the progression of hepatocellular carcinoma.

Authors - Yilu Feng
human,mouseIHC,IF

Sci Total Environ

Alpha-ketoglutarate alleviates cadmium-induced inflammation by inhibiting the HIF1A-TNFAIP3 pathway in hepatocytes

Authors - Yinzhao Jia
humanWB

Biochim Biophys Acta Mol Basis Dis

Rhythm gene PER1 mediates ferroptosis and lipid metabolism through SREBF2/ALOX15 axis in polycystic ovary syndrome

Authors - Yuanyuan Chen