TNFAIP3 Monoclonal antibody
TNFAIP3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3A11G6
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells |
Positive IHC detected in | mouse kidney tissue, human thyroid cancer tissue, rat ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66695-1-Ig targets TNFAIP3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | TNFAIP3 fusion protein Ag18150 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | tumor necrosis factor, alpha-induced protein 3 |
Synonyms | OTUD7C, OTU domain-containing protein 7C, EC:2.3.2.-, A20p50, A20p37 |
Calculated Molecular Weight | 790 aa, 90 kDa |
Observed Molecular Weight | 80 kDa |
GenBank Accession Number | BC114480 |
Gene Symbol | TNFAIP3 |
Gene ID (NCBI) | 7128 |
RRID | AB_2882048 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P21580 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TNFAIP3 antibody 66695-1-Ig | Download protocol |
IHC protocol for TNFAIP3 antibody 66695-1-Ig | Download protocol |
IF protocol for TNFAIP3 antibody 66695-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biology (Basel) Interleukin-10 Deficiency Impacts on TNF-Induced NFκB Regulated Responses In Vivo | ||
Cell Death Dis A20 targets PFKL and glycolysis to inhibit the progression of hepatocellular carcinoma. | ||
Sci Total Environ Alpha-ketoglutarate alleviates cadmium-induced inflammation by inhibiting the HIF1A-TNFAIP3 pathway in hepatocytes | ||
Biochim Biophys Acta Mol Basis Dis Rhythm gene PER1 mediates ferroptosis and lipid metabolism through SREBF2/ALOX15 axis in polycystic ovary syndrome |