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验证数据展示

  • WB analysis using 66821-1-Ig (same clone as 66821-1-PBS)

    WB analysis using 66821-1-Ig (same clone as 66821-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66821-1-Ig (Syntaxin 10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66821-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66821-1-Ig (same clone as 66821-1-PBS)

    IF Staining of HeLa using 66821-1-Ig (same clone as 66821-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Syntaxin 10 antibody (66821-1-Ig, Clone: 1B1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66821-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51170-3

    Cytometric bead array standard curve of MP51170-3

    Cytometric bead array standard curve of MP51170-3, STX10 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66821-5-PBS. Detection antibody: 66821-1-PBS. Standard:Ag27861. Range: 1.563-100 ng/mL.

Syntaxin 10 Monoclonal antibody, PBS Only (Detector)

Syntaxin 10 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66821-1-PBS

产品说明书

CloneNo. 1B1C3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Syntaxin-10, Syn10, STX10, 1B1C3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy STX10 Ready-To-Use IHC Kit. STX10 primary antibody included.

产品信息

66821-1-PBS targets Syntaxin 10 as part of a matched antibody pair:

MP51170-3: 66821-5-PBS capture and 66821-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Syntaxin 10 fusion protein Ag27861 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 syntaxin 10
别名 Syntaxin-10, Syn10, STX10, 1B1C3
计算分子量 28 kDa
观测分子量 34 kDa
GenBank蛋白编号BC017237
基因名称 Syntaxin 10
Gene ID (NCBI) 8677
RRIDAB_2882164
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60499
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Syntaxin 10 (STX10), also named as SYN10, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes.