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VEGFA Monoclonal antibody
VEGFA Monoclonal Antibody for WB, IF/ICC,IF-P, IHC, ELISA, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
Human and More (5)
Applications
WB, IF/ICC,IF-P, IHC, ELISA, FC (Intra)
Conjugate
Unconjugated
104
CloneNo.
2E2H9
验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HUVEC cells, NCI-H1299 cells, A431 cells, SH-SY5Y cells, HuH-7 cells, T-47D cells, MDA-MB-231 cells |
Positive IHC detected in | human lung cancer tissue, human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells, human endometrial cancer tissue, human lung cancer tissue |
Positive FC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66828-1-Ig targets VEGFA in WB, IF, IHC, ELISA, FC (Intra) applications and shows reactivity with Human samples.
Tested Applications | WB, IF/ICC,IF-P, IHC, ELISA, FC (Intra) Application Description |
Cited Applications | WB, IF, IHC, ELISA |
Tested Reactivity | Human |
Cited Reactivity | human, rat, goat, zebrafish, pig |
Immunogen | VEGFA fusion protein Ag13500 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | vascular endothelial growth factor A |
Synonyms | L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF |
Calculated Molecular Weight | 412 aa, 46 kDa |
Observed Molecular Weight | 43 kDa |
GenBank Accession Number | BC065522 |
Gene Symbol | VEGFA |
Gene ID (NCBI) | 7422 |
RRID | AB_2882171 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P15692 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for VEGFA antibody 66828-1-Ig | Download protocol |
IHC protocol for VEGFA antibody 66828-1-Ig | Download protocol |
IF protocol for VEGFA antibody 66828-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single-Cell Resolution | ||
Redox Biol Nox2 impairs VEGF-A-induced angiogenesis in placenta via mitochondrial ROS-STAT3 pathway.
| ||
Oxid Med Cell Longev Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway. | ||
Mol Ther Nucleic Acids circ_2858 Helps Blood-Brain Barrier Disruption by Increasing VEGFA via Sponging miR-93-5p during Escherichia coli Meningitis. | ||
Bioengineered LEM domain containing 1 (LEMD1) transcriptionally activated by SRY-related high-mobility-group box 4 (SOX4) accelerates the progression of colon cancer by upregulating phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway |