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验证数据展示

  • IF Staining of HeLa using CL594-66828

    IF Staining of HeLa using CL594-66828

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 VEGFA antibody (CL594-66828, Clone: 2E2H9 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL594-66828

    FC experiment of MCF-7 using CL594-66828

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated VEGFA Monoclonal antibody (CL594-66828, Clone:2E2H9)(red), or 0.4 ug Control Antibody (blue). Cells were not fixed.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated VEGFA Monoclonal antibody

VEGFA Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66828

产品说明书

CloneNo. 2E2H9

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

VEGF, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy VEGFA/VEGF Ready-To-Use IHC Kit. VEGFA/VEGF primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66828-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66828 targets VEGFA in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 VEGFA fusion protein Ag13500 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 vascular endothelial growth factor A
别名 VEGF, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF
计算分子量 412 aa, 46 kDa
观测分子量 43 kDa
GenBank蛋白编号BC065522
基因名称 VEGFA
Gene ID (NCBI) 7422
RRIDAB_3084729
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP15692
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38kDa or VEFGA110 34kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.

实验方案

Product Specific Protocols
IF protocol for CL594 VEGFA antibody CL594-66828Download protocol
Standard Protocols
Click here to view our Standard Protocols