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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated VEGFA Monoclonal antibody

VEGFA Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E2H9

Cat no : CL594-66828

Print datasheet

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

验证数据展示

  • IF Staining of HeLa using CL594-66828

    IF Staining of HeLa using CL594-66828

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 VEGFA antibody (CL594-66828, Clone: 2E2H9 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL594-66828

    FC experiment of MCF-7 using CL594-66828

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated VEGFA Monoclonal antibody (CL594-66828, Clone:2E2H9)(red), or 0.4 ug Control Antibody (blue). Cells were not fixed.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy VEGFA/VEGF Ready-To-Use IHC Kit. VEGFA/VEGF primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66828-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66828 targets VEGFA in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human
Immunogen VEGFA fusion protein Ag13500 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name vascular endothelial growth factor A
Synonyms L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF
Calculated Molecular Weight 412 aa, 46 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC065522
Gene Symbol VEGFA
Gene ID (NCBI) 7422
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15692
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38kDa or VEFGA110 34kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.

实验方案

Product Specific Protocols
IF protocol for CL594 VEGFA antibody CL594-66828Download protocol
Standard Protocols
Click here to view our Standard Protocols