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验证数据展示

  • WB analysis of MCF-7 using 66828-1-Ig

    WB analysis of MCF-7 using 66828-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 66828-1-Ig

    IHC staining of human lung cancer using 66828-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VEGFA/VEGF antibody included
  • IHC staining of human lung cancer using 66828-1-Ig

    IHC staining of human lung cancer using 66828-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VEGFA/VEGF antibody included
  • IF Staining of MCF-7 using 66828-1-Ig

    IF Staining of MCF-7 using 66828-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human endometrial cancer using 66828-1-Ig

    IF Staining of human endometrial cancer using 66828-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human endometrial cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of MCF-7 using 66828-1-Ig

    FC experiment of MCF-7 using 66828-1-Ig

    1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human VEGFA (66828-1-Ig, Clone:2E2H9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

VEGFA Monoclonal antibody

VEGFA Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 66828-1-Ig

产品说明书

CloneNo. 2E2H9
引用文献(123)

宿主/亚型

Mouse / IgG2b

种属反应性

human and More (4)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA and More (1)

别名

VEGF, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HUVEC cells, NCI-H1299 cells, A431 cells, SH-SY5Y cells, HuH-7 cells, T-47D cells, MDA-MB-231 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue, human liver cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman endometrial cancer tissue, human lung cancer tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy VEGFA/VEGF Ready-To-Use IHC Kit. VEGFA/VEGF primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66828-1-Ig targets VEGFA in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF, ELISA
经测试反应性 human
文献引用反应性human, rat, pig, zebrafish, goat
免疫原 VEGFA fusion protein Ag13500 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 vascular endothelial growth factor A
别名 VEGF, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF
计算分子量 412 aa, 46 kDa
观测分子量 43 kDa
GenBank蛋白编号BC065522
基因名称 VEGFA
Gene ID (NCBI) 7422
RRIDAB_2882171
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP15692
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.

实验方案

Product Specific Protocols
WB protocol for VEGFA antibody 66828-1-IgDownload protocol
IHC protocol for VEGFA antibody 66828-1-IgDownload protocol
IF protocol for VEGFA antibody 66828-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Adv Sci (Weinh)

Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single-Cell Resolution

Authors - Taifeng Zhou
View Article
pigWB

Redox Biol

Nox2 impairs VEGF-A-induced angiogenesis in placenta via mitochondrial ROS-STAT3 pathway.

Authors - Chengjun Hu
  • KD Validated
View Article
humanWB

J Transl Med

KPNA2 promotes angiogenesis by regulating STAT3 phosphorylation

Authors - Yujie Jia
View Article
humanWB

Oxid Med Cell Longev

Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway.

Authors - Ziwei Yang
View Article
humanWB

Mol Ther Nucleic Acids

circ_2858 Helps Blood-Brain Barrier Disruption by Increasing VEGFA via Sponging miR-93-5p during Escherichia coli Meningitis.

Authors - Ruicheng Yang
View Article
humanWB

Bioengineered

LEM domain containing 1 (LEMD1) transcriptionally activated by SRY-related high-mobility-group box 4 (SOX4) accelerates the progression of colon cancer by upregulating phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway

Authors - Ding Li
View Article