验证数据展示
经过测试的应用
| Positive WB detected in | pig kidney tissue, pig liver tissue, rat liver tissue |
| Positive IHC detected in | mouse kidney tissue, human kidney tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PCK1 Ready-To-Use IHC Kit. PCK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
66862-1-Ig targets PCK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human, Mouse, Rat, Pig |
| 文献引用反应性 | monkey |
| 免疫原 | PCK1 fusion protein Ag9720 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | phosphoenolpyruvate carboxykinase 1 (soluble) |
| 别名 | PCK1, PEPCK C, PEPCK1, PEPCKC |
| 计算分子量 | 622 aa, 69 kDa |
| 观测分子量 | 65-69 kDa |
| GenBank蛋白编号 | BC023978 |
| 基因名称 | PCK1 |
| Gene ID (NCBI) | 5105 |
| RRID | AB_2882200 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P35558 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PCK1 antibody 66862-1-Ig | Download protocol |
| IHC protocol for PCK1 antibody 66862-1-Ig | Download protocol |
| IF protocol for PCK1 antibody 66862-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
