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PCK1 Monoclonal antibody, PBS Only

PCK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F11B5

Cat no : 66862-1-PBS

Print datasheet

Synonyms

PCK1, PEPCK C, PEPCK1, PEPCKC

验证数据展示

  • WB analysis using 66862-1-Ig (same clone as 66862-1-PBS)

    WB analysis using 66862-1-Ig (same clone as 66862-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66862-1-Ig (PCK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66862-1-Ig (same clone as 66862-1-PBS)

    IF Staining of HeLa using 66862-1-Ig (same clone as 66862-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PCK1 antibody (66862-1-Ig, Clone: 2F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

    IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCK1 antibody included
  • IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

    IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCK1 antibody included
Planning an IHC experiment? We recommend our IHCeasy PCK1 Ready-To-Use IHC Kit. PCK1 primary antibody included.

产品信息

66862-1-PBS targets PCK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen PCK1 fusion protein Ag9720 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphoenolpyruvate carboxykinase 1 (soluble)
Synonyms PCK1, PEPCK C, PEPCK1, PEPCKC
Calculated Molecular Weight 622 aa, 69 kDa
Observed Molecular Weight 65-69 kDa
GenBank Accession NumberBC023978
Gene Symbol PCK1
Gene ID (NCBI) 5105
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35558
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).