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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 66862-1-Ig (same clone as 66862-1-PBS)

    WB analysis using 66862-1-Ig (same clone as 66862-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66862-1-Ig (PCK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66862-1-Ig (same clone as 66862-1-PBS)

    IF Staining of HeLa using 66862-1-Ig (same clone as 66862-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PCK1 antibody (66862-1-Ig, Clone: 2F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

    IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCK1 antibody included
  • IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

    IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCK1 antibody included

PCK1 Monoclonal antibody, PBS Only

PCK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66862-1-PBS

产品说明书

CloneNo. 2F11B5

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IHC, IF/ICC, ELISA

别名

PCK1, PEPCK C, PEPCK1, PEPCKC

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PCK1 Ready-To-Use IHC Kit. PCK1 primary antibody included.

产品信息

66862-1-PBS targets PCK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 PCK1 fusion protein Ag9720 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoenolpyruvate carboxykinase 1 (soluble)
别名 PCK1, PEPCK C, PEPCK1, PEPCKC
计算分子量 622 aa, 69 kDa
观测分子量 65-69 kDa
GenBank蛋白编号BC023978
基因名称 PCK1
Gene ID (NCBI) 5105
RRIDAB_2882200
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP35558
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).