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PCK1 Monoclonal antibody, PBS Only

PCK1 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

2F11B5

Cat No : 66862-1-PBS

Print datasheet

Synonyms

PCK1, PEPCK C, PEPCK1, PEPCKC

验证数据展示

  • WB analysis using 66862-1-Ig (same clone as 66862-1-PBS)

    WB analysis using 66862-1-Ig (same clone as 66862-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66862-1-Ig (PCK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66862-1-Ig (same clone as 66862-1-PBS)

    IF Staining of HeLa using 66862-1-Ig (same clone as 66862-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PCK1 antibody (66862-1-Ig, Clone: 2F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

    IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCK1 antibody included
  • IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

    IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCK1 antibody included
Planning an IHC experiment? We recommend our IHCeasy PCK1 Ready-To-Use IHC Kit. PCK1 primary antibody included.

产品信息

66862-1-PBS targets PCK1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen PCK1 fusion protein Ag9720 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphoenolpyruvate carboxykinase 1 (soluble)
Synonyms PCK1, PEPCK C, PEPCK1, PEPCKC
Calculated Molecular Weight 622 aa, 69 kDa
Observed Molecular Weight 65-69 kDa
GenBank Accession NumberBC023978
Gene Symbol PCK1
Gene ID (NCBI) 5105
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35558
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).