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PCK1 Monoclonal antibody

PCK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F11B5

Cat no : 66862-1-Ig

Print datasheet

Synonyms

PCK1, PEPCK C, PEPCK1, PEPCKC

验证数据展示

  • WB analysis using 66862-1-Ig

    WB analysis using 66862-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66862-1-Ig (PCK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66862-1-Ig

    IF Staining of HeLa using 66862-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PCK1 antibody (66862-1-Ig, Clone: 2F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of mouse kidney using 66862-1-Ig

    IHC staining of mouse kidney using 66862-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCK1 antibody included
  • IHC staining of mouse kidney using 66862-1-Ig

    IHC staining of mouse kidney using 66862-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PCK1 antibody included

经过测试的应用

Positive WB detected inpig kidney tissue, pig liver tissue, rat liver tissue
Positive IHC detected inmouse kidney tissue, human kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PCK1 Ready-To-Use IHC Kit. PCK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66862-1-Ig targets PCK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen PCK1 fusion protein Ag9720 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphoenolpyruvate carboxykinase 1 (soluble)
Synonyms PCK1, PEPCK C, PEPCK1, PEPCKC
Calculated Molecular Weight 622 aa, 69 kDa
Observed Molecular Weight 65-69 kDa
GenBank Accession NumberBC023978
Gene Symbol PCK1
Gene ID (NCBI) 5105
RRIDAB_2882200
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35558
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).

实验方案

Product Specific Protocols
WB protocol for PCK1 antibody 66862-1-IgDownload protocol
IHC protocol for PCK1 antibody 66862-1-IgDownload protocol
IF protocol for PCK1 antibody 66862-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols