CoraLite® Plus 488-conjugated PCK1 Monoclonal antibody
PCK1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
mouse, rat, pig, human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2F11B5
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PCK1 Ready-To-Use IHC Kit. PCK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66862-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66862 targets PCK1 in IF/ICC applications and shows reactivity with mouse, rat, pig, human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | mouse, rat, pig, human |
| Immunogen | PCK1 fusion protein Ag9720 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | phosphoenolpyruvate carboxykinase 1 (soluble) |
| Synonyms | PCK1, PEPCK C, PEPCK1, PEPCKC |
| Calculated Molecular Weight | 622 aa, 69 kDa |
| Observed Molecular Weight | 65-69 kDa |
| GenBank Accession Number | BC023978 |
| Gene Symbol | PCK1 |
| Gene ID (NCBI) | 5105 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P35558 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PCK1 antibody CL488-66862 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |