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LXN Monoclonal antibody

LXN Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3E5A5

Cat no : 66957-1-Ig

Print datasheet

Synonyms

TCI, Protein MUM, Latexin, ECI, 3E5A5

验证数据展示

  • WB analysis of pig brain using 66957-1-Ig

    WB analysis of pig brain using 66957-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 66957-1-Ig

    IHC staining of human heart using 66957-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66957-1-Ig (LXN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human heart using 66957-1-Ig

    IHC staining of human heart using 66957-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66957-1-Ig (LXN antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66957-1-Ig

    IF Staining of HeLa using 66957-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LXN antibody (66957-1-Ig, Clone: 3E5A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 66957-1-Ig

    FC experiment of HeLa using 66957-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LXN (66957-1-Ig, Clone:3E5A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inpig brain tissue, A549 cells, HEK-293 cells, SH-SY5Y cells, rabbit brain tissue, HeLa cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66957-1-Ig targets LXN in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, pig, rabbit
Immunogen LXN fusion protein Ag27922 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name latexin
Synonyms TCI, Protein MUM, Latexin, ECI, 3E5A5
Calculated Molecular Weight 222 aa, 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC005346
Gene Symbol LXN
Gene ID (NCBI) 56925
RRIDAB_2882280
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BS40
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Latexin, also named as LTXN, LXN, ECI, MUM and TCI, is hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. It may function as a neuroprotective mechanism against tau toxicity.

实验方案

Product Specific Protocols
WB protocol for LXN antibody 66957-1-IgDownload protocol
IHC protocol for LXN antibody 66957-1-IgDownload protocol
IF protocol for LXN antibody 66957-1-IgDownload protocol
FC protocol for LXN antibody 66957-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols