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PLCD1 Monoclonal antibody

PLCD1 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D10B11

Cat no : 67064-1-Ig

Print datasheet

Synonyms

Phospholipase C III, Phospholipase C delta 1, Phosphoinositide phospholipase C-delta-1, EC:3.1.4.11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1

验证数据展示

  • WB analysis of human testis using 67064-1-Ig

    WB analysis of human testis using 67064-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 67064-1-Ig (PLCD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 67064-1-Ig

    IHC staining of human testis using 67064-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 67064-1-Ig (PLCD1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 67064-1-Ig

    IHC staining of human testis using 67064-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 67064-1-Ig (PLCD1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of K-562 using 67064-1-Ig

    FC experiment of K-562 using 67064-1-Ig

    1x10^6 K-562 cells were intracellularly stained with 0.2 ug PLCD1 Monoclonal antibody (67064-1-Ig, Clone:1D10B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inhuman testis tissue, K-562 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67064-1-Ig targets PLCD1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen PLCD1 fusion protein Ag5567 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phospholipase C, delta 1
Synonyms Phospholipase C III, Phospholipase C delta 1, Phosphoinositide phospholipase C-delta-1, EC:3.1.4.11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC050382
Gene Symbol PLCD1
Gene ID (NCBI) 5333
RRIDAB_2882374
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51178
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLCD1 antibody 67064-1-IgDownload protocol
IHC protocol for PLCD1 antibody 67064-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols