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PLCD1 Monoclonal antibody, PBS Only

PLCD1 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D10B11

Cat no : 67064-1-PBS

Print datasheet

Synonyms

Phospholipase C III, Phospholipase C delta 1, Phosphoinositide phospholipase C-delta-1, EC:3.1.4.11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1

验证数据展示

  • WB analysis of human testis using 67064-1-Ig (same clone as 67064-1-PBS)

    WB analysis of human testis using 67064-1-Ig (same clone as 67064-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 67064-1-Ig (PLCD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67064-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 67064-1-Ig (same clone as 67064-1-PBS)

    IHC staining of human testis using 67064-1-Ig (same clone as 67064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 67064-1-Ig (PLCD1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67064-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 67064-1-Ig (same clone as 67064-1-PBS)

    IHC staining of human testis using 67064-1-Ig (same clone as 67064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 67064-1-Ig (PLCD1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67064-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 67064-1-Ig (same clone as 67064-1-PBS)

    FC experiment of K-562 using 67064-1-Ig (same clone as 67064-1-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.2 ug PLCD1 Monoclonal antibody (67064-1-Ig, Clone:1D10B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67064-1-PBS in a different storage buffer formulation.

产品信息

67064-1-PBS targets PLCD1 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen PLCD1 fusion protein Ag5567 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phospholipase C, delta 1
Synonyms Phospholipase C III, Phospholipase C delta 1, Phosphoinositide phospholipase C-delta-1, EC:3.1.4.11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC050382
Gene Symbol PLCD1
Gene ID (NCBI) 5333
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51178
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C