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INA Monoclonal antibody

INA Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3C12A8

Cat no : 67120-1-Ig

Print datasheet

Synonyms

66 kDa neurofilament protein, Alpha internexin, Alpha Inx, FLJ18662, FLJ57501, INA, NEF5, Neurofilament 5, Neurofilament 66, NF 66, TXBP 1

验证数据展示

  • WB analysis using 67120-1-Ig

    WB analysis using 67120-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67120-1-Ig (INA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67120-1-Ig

    WB analysis using 67120-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67120-1-Ig (INA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 67120-1-Ig

    IHC staining of human pancreas cancer using 67120-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67120-1-Ig (INA antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected infetal human brain tissue, rat cerebellum tissue, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67120-1-Ig targets INA in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen INA fusion protein Ag13655 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name internexin neuronal intermediate filament protein, alpha
Synonyms 66 kDa neurofilament protein, Alpha internexin, Alpha Inx, FLJ18662, FLJ57501, INA, NEF5, Neurofilament 5, Neurofilament 66, NF 66, TXBP 1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 60-66 kDa
GenBank Accession NumberBC006359
Gene Symbol INA
Gene ID (NCBI) 9118
RRIDAB_2882423
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16352
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INA, also named as Alpha internexin, is a 66 kDa intermediate filament protein which is involved in the morphogenesis of neurons. INA is mainly expressed in various kinds of central and peripheral neurons from early development and highly expressed in neurodegenerative and neuronal death cells. The MW of INA ranges from 60 kDa in human, pig and mouse to 66 kDa in rat.(PMID: 29940892, 2201753)

实验方案

Product Specific Protocols
WB protocol for INA antibody 67120-1-IgDownload protocol
IHC protocol for INA antibody 67120-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols