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验证数据展示

  • WB analysis using 67120-1-Ig (same clone as 67120-1-PBS)

    WB analysis using 67120-1-Ig (same clone as 67120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67120-1-Ig (INA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

  • WB analysis using 67120-1-Ig (same clone as 67120-1-PBS)

    WB analysis using 67120-1-Ig (same clone as 67120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67120-1-Ig (INA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 67120-1-Ig (same clone as 67120-1-PBS)

    IF Staining of SH-SY5Y using 67120-1-Ig (same clone as 67120-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using INA antibody (67120-1-Ig, Clone: 3C12A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67120-1-Ig (same clone as 67120-1-PBS)

    IHC staining of human pancreas cancer using 67120-1-Ig (same clone as 67120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67120-1-Ig (INA antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

INA Monoclonal antibody, PBS Only

INA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67120-1-PBS

产品说明书

CloneNo. 3C12A8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Alpha internexin, 66 kDa neurofilament protein, NEF5, Alpha-Inx, Alpha-internexin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67120-1-PBS targets INA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 INA fusion protein Ag13655 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 internexin neuronal intermediate filament protein, alpha
别名 Alpha internexin, 66 kDa neurofilament protein, NEF5, Alpha-Inx, Alpha-internexin
计算分子量 55 kDa
观测分子量 60-66 kDa
GenBank蛋白编号BC006359
基因名称 INA
Gene ID (NCBI) 9118
RRIDAB_2882423
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16352
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

INA, also named as Alpha internexin, is a 66 kDa intermediate filament protein which is involved in the morphogenesis of neurons. INA is mainly expressed in various kinds of central and peripheral neurons from early development and highly expressed in neurodegenerative and neuronal death cells. The MW of INA ranges from 60 kDa in human, pig and mouse to 66 kDa in rat.(PMID: 29940892, 2201753)