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INA Monoclonal antibody, PBS Only

INA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C12A8

Cat no : 67120-1-PBS

Print datasheet

Synonyms

Alpha internexin, 66 kDa neurofilament protein, NEF5, Alpha-Inx, Alpha-internexin

验证数据展示

  • WB analysis using 67120-1-Ig (same clone as 67120-1-PBS)

    WB analysis using 67120-1-Ig (same clone as 67120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67120-1-Ig (INA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

  • WB analysis using 67120-1-Ig (same clone as 67120-1-PBS)

    WB analysis using 67120-1-Ig (same clone as 67120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67120-1-Ig (INA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 67120-1-Ig (same clone as 67120-1-PBS)

    IF Staining of SH-SY5Y using 67120-1-Ig (same clone as 67120-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using INA antibody (67120-1-Ig, Clone: 3C12A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67120-1-Ig (same clone as 67120-1-PBS)

    IHC staining of human pancreas cancer using 67120-1-Ig (same clone as 67120-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67120-1-Ig (INA antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67120-1-PBS in a different storage buffer formulation.

产品信息

67120-1-PBS targets INA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen INA fusion protein Ag13655 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name internexin neuronal intermediate filament protein, alpha
Synonyms Alpha internexin, 66 kDa neurofilament protein, NEF5, Alpha-Inx, Alpha-internexin
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 60-66 kDa
GenBank Accession NumberBC006359
Gene Symbol INA
Gene ID (NCBI) 9118
RRIDAB_2882423
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16352
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

INA, also named as Alpha internexin, is a 66 kDa intermediate filament protein which is involved in the morphogenesis of neurons. INA is mainly expressed in various kinds of central and peripheral neurons from early development and highly expressed in neurodegenerative and neuronal death cells. The MW of INA ranges from 60 kDa in human, pig and mouse to 66 kDa in rat.(PMID: 29940892, 2201753)