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验证数据展示

  • WB analysis using 67120-1-Ig

    WB analysis using 67120-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67120-1-Ig (INA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67120-1-Ig

    WB analysis using 67120-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67120-1-Ig (INA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 67120-1-Ig

    IF Staining of SH-SY5Y using 67120-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using INA antibody (67120-1-Ig, Clone: 3C12A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IHC staining of human pancreas cancer using 67120-1-Ig

    IHC staining of human pancreas cancer using 67120-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67120-1-Ig (INA antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

INA Monoclonal antibody

INA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67120-1-Ig

产品说明书

CloneNo. 3C12A8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA

别名

Alpha internexin, 66 kDa neurofilament protein, NEF5, Alpha-Inx, Alpha-internexin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected infetal human brain tissue, rat cerebellum tissue, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67120-1-Ig targets INA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 INA fusion protein Ag13655 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 internexin neuronal intermediate filament protein, alpha
别名 Alpha internexin, 66 kDa neurofilament protein, NEF5, Alpha-Inx, Alpha-internexin
计算分子量 55 kDa
观测分子量 60-66 kDa
GenBank蛋白编号BC006359
基因名称 INA
Gene ID (NCBI) 9118
RRIDAB_2882423
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16352
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INA, also named as Alpha internexin, is a 66 kDa intermediate filament protein which is involved in the morphogenesis of neurons. INA is mainly expressed in various kinds of central and peripheral neurons from early development and highly expressed in neurodegenerative and neuronal death cells. The MW of INA ranges from 60 kDa in human, pig and mouse to 66 kDa in rat.(PMID: 29940892, 2201753)

实验方案

Product Specific Protocols
WB protocol for INA antibody 67120-1-IgDownload protocol
IHC protocol for INA antibody 67120-1-IgDownload protocol
IF protocol for INA antibody 67120-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols