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验证数据展示

  • IF Staining of SH-SY5Y using CL594-67120

    IF Staining of SH-SY5Y using CL594-67120

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite®594 INA antibody (CL594-67120, Clone: 3C12A8 ) at dilution of 1:200.

  • FC experiment of SH-SY5Y using CL594-67120

    FC experiment of SH-SY5Y using CL594-67120

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human INA (CL594-67120, Clone:3C12A8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated INA Monoclonal antibody

INA Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67120

产品说明书

CloneNo. 3C12A8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

Alpha internexin, 66 kDa neurofilament protein, NEF5, Alpha-Inx, Alpha-internexin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
For other applications, we recommend the unconjugated version of this antibody, 67120-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67120 targets INA in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 INA fusion protein Ag13655 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 internexin neuronal intermediate filament protein, alpha
别名 Alpha internexin, 66 kDa neurofilament protein, NEF5, Alpha-Inx, Alpha-internexin
计算分子量 55 kDa
观测分子量 60-66 kDa
GenBank蛋白编号BC006359
基因名称 INA
Gene ID (NCBI) 9118
RRIDAB_2934757
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16352
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INA, also named as Alpha internexin, is a 66 kDa intermediate filament protein which is involved in the morphogenesis of neurons. INA is mainly expressed in various kinds of central and peripheral neurons from early development and highly expressed in neurodegenerative and neuronal death cells. The MW of INA ranges from 60 kDa in human, pig and mouse to 66 kDa in rat.(PMID: 29940892, 2201753)

实验方案

Product Specific Protocols
IF protocol for CL594 INA antibody CL594-67120Download protocol
Standard Protocols
Click here to view our Standard Protocols