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验证数据展示

  • WB analysis of NIH/3T3 using 67359-1-Ig

    WB analysis of NIH/3T3 using 67359-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 67359-1-Ig

    FC experiment of HEK-293 using 67359-1-Ig

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug RALBP1 Monoclonal antibody (67359-1-Ig, Clone:1B2F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

RALBP1 Monoclonal antibody

RALBP1 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67359-1-Ig

产品说明书

CloneNo. 1B2F5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

DNP-SG ATPase, DNP SG ATPase, Dinitrophenyl S-glutathione ATPase, 76 kDa Ral-interacting protein, 76 kDa Ral interacting protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, Jurkat cells, HEK-293 cells, LNCaP cells, MCF-7 cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy RALBP1 Ready-To-Use IHC Kit. RALBP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67359-1-Ig targets RALBP1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 RALBP1 fusion protein Ag28618 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ralA binding protein 1
别名 DNP-SG ATPase, DNP SG ATPase, Dinitrophenyl S-glutathione ATPase, 76 kDa Ral-interacting protein, 76 kDa Ral interacting protein
计算分子量 655 aa, 76 kDa
观测分子量 95 kDa
GenBank蛋白编号BC013126
基因名称 RALBP1
Gene ID (NCBI) 10928
RRIDAB_2882611
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15311
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RALBP1 antibody 67359-1-IgDownload protocol
FC protocol for RALBP1 antibody 67359-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols