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验证数据展示

  • IF Staining of HepG2 using 67425-1-Ig

    IF Staining of HepG2 using 67425-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:1500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • IF Staining of HeLa using 67425-1-Ig

    IF Staining of HeLa using 67425-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Sam50 Monoclonal antibody

Sam50 Monoclonal Antibody for IF/ICC, ELISA
Cat No. 67425-1-Ig

产品说明书

CloneNo. 2H6H9
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human and More (1)

应用

IF/ICC, ELISA and More (1)

别名

SAMM50, SAMM 50, OMP85, CGI-51, CGI 51

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells, HepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

67425-1-Ig targets Sam50 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human
文献引用反应性mouse
免疫原 Sam50 fusion protein Ag15155 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sorting and assembly machinery component 50 homolog (S. cerevisiae)
别名 SAMM50, SAMM 50, OMP85, CGI-51, CGI 51
计算分子量 469 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC007830
基因名称 Sam50
Gene ID (NCBI) 25813
RRIDAB_2882664
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y512
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

实验方案

Product Specific Protocols
IF protocol for Sam50 antibody 67425-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Acta Pharm Sin B

Novel Pt(IV) complex OAP2 induces STING activation and pyroptosis via mitochondrial membrane remodeling for synergistic chemo-immunotherapy

Authors - Renming Fan
View Article