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Sam50 Monoclonal antibody, PBS Only

Sam50 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat, Mouse

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

2H6H9

Cat No : 67425-1-PBS

Print datasheet

Synonyms

CGI 51, FLJ35825, FLJ42905, FLJ99036, OMP85, Sam50, SAMM50, TOB55, TRG 3, TRG3, YNL026W

验证数据展示

  • WB analysis using 67425-1-Ig (same clone as 67425-1-PBS)

    WB analysis using 67425-1-Ig (same clone as 67425-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67425-1-Ig (Sam50 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67425-1-Ig (same clone as 67425-1-PBS)

    IHC staining of human liver cancer using 67425-1-Ig (same clone as 67425-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67425-1-Ig (SAMM50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67425-1-Ig (same clone as 67425-1-PBS)

    IHC staining of human liver cancer using 67425-1-Ig (same clone as 67425-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67425-1-Ig (SAMM50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67425-1-Ig (same clone as 67425-1-PBS)

    IF Staining of HeLa using 67425-1-Ig (same clone as 67425-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

产品信息

67425-1-PBS targets Sam50 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig, Rat, Mouse samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity Human, Pig, Rat, Mouse
Immunogen Sam50 fusion protein Ag15155 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sorting and assembly machinery component 50 homolog (S. cerevisiae)
Synonyms CGI 51, FLJ35825, FLJ42905, FLJ99036, OMP85, Sam50, SAMM50, TOB55, TRG 3, TRG3, YNL026W
Calculated Molecular Weight 469 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC007830
Gene Symbol SAMM50
Gene ID (NCBI) 25813
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y512
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).