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Sam50 Monoclonal antibody, PBS Only

Sam50 Monoclonal Antibody for IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H6H9

Cat no : 67425-1-PBS

Print datasheet

Synonyms

SAMM50, SAMM 50, OMP85, CGI-51, CGI 51

验证数据展示

  • IF Staining of HepG2 using 67425-1-Ig (same clone as 67425-1-PBS)

    IF Staining of HepG2 using 67425-1-Ig (same clone as 67425-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:1500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67425-1-Ig (same clone as 67425-1-PBS)

    IF Staining of HeLa using 67425-1-Ig (same clone as 67425-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

产品信息

67425-1-PBS targets Sam50 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Sam50 fusion protein Ag15155 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sorting and assembly machinery component 50 homolog (S. cerevisiae)
Synonyms SAMM50, SAMM 50, OMP85, CGI-51, CGI 51
Calculated Molecular Weight 469 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC007830
Gene Symbol Sam50
Gene ID (NCBI) 25813
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y512
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).