验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells, HepG2 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
67425-1-Ig targets Sam50 in IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | IF/ICC, ELISA Application Description |
Cited Applications | IF |
Tested Reactivity | human |
Cited Reactivity | mouse |
Immunogen | Sam50 fusion protein Ag15155 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | sorting and assembly machinery component 50 homolog (S. cerevisiae) |
Synonyms | SAMM50, SAMM 50, OMP85, CGI-51, CGI 51 |
Calculated Molecular Weight | 469 aa, 52 kDa |
Observed Molecular Weight | 52 kDa |
GenBank Accession Number | BC007830 |
Gene Symbol | Sam50 |
Gene ID (NCBI) | 25813 |
RRID | AB_2882664 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9Y512 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).
实验方案
Product Specific Protocols | |
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IF protocol for Sam50 antibody 67425-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |