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CoraLite®594-conjugated Sam50 Monoclonal antibody

Sam50 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat, Mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2H6H9

Cat no : CL594-67425

Print datasheet

Synonyms

CGI 51, FLJ35825, FLJ42905, FLJ99036, OMP85, SAM50, SAMM50, TOB55, TRG 3, TRG3, YNL026W

验证数据展示

  • IF Staining of HepG2 using CL594-67425

    IF Staining of HepG2 using CL594-67425

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated Sam50 antibody (CL594-67425, Clone: 2H6H9 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67425-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67425 targets Sam50 in IF/ICC applications and shows reactivity with Human, Pig, Rat, Mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Pig, Rat, Mouse
Immunogen Sam50 fusion protein Ag15155 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sorting and assembly machinery component 50 homolog (S. cerevisiae)
Synonyms CGI 51, FLJ35825, FLJ42905, FLJ99036, OMP85, SAM50, SAMM50, TOB55, TRG 3, TRG3, YNL026W
Calculated Molecular Weight 469 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC007830
Gene Symbol Sam50
Gene ID (NCBI) 25813
RRIDAB_2920133
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y512
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

实验方案

Product Specific Protocols
IF protocol for CL594 Sam50 antibody CL594-67425Download protocol
Standard Protocols
Click here to view our Standard Protocols