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验证数据展示

  • IF Staining of HepG2 using CL594-67425

    IF Staining of HepG2 using CL594-67425

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated Sam50 antibody (CL594-67425, Clone: 2H6H9 ) at dilution of 1:100.

CoraLite®594-conjugated Sam50 Monoclonal antibody

Sam50 Monoclonal Antibody for IF/ICC
Cat No. CL594-67425

产品说明书

CloneNo. 2H6H9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Pig, Rat, Mouse

应用

IF/ICC

别名

CGI 51, FLJ35825, FLJ42905, FLJ99036, OMP85, SAM50, SAMM50, TOB55, TRG 3, TRG3, YNL026W

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594

规格: 
100 μL

¥2800/ 100 μL

浓度: 1000 μg/ml

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67425-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67425 targets Sam50 in IF/ICC applications and shows reactivity with Human, Pig, Rat, Mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Pig, Rat, Mouse
免疫原 Sam50 fusion protein Ag15155 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sorting and assembly machinery component 50 homolog (S. cerevisiae)
别名 CGI 51, FLJ35825, FLJ42905, FLJ99036, OMP85, SAM50, SAMM50, TOB55, TRG 3, TRG3, YNL026W
计算分子量 469 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC007830
基因名称 Sam50
Gene ID (NCBI) 25813
RRIDAB_2920133
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y512
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

实验方案

Product Specific Protocols
IF protocol for CL594 Sam50 antibody CL594-67425Download protocol
Standard Protocols
Click here to view our Standard Protocols