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MTH1 Monoclonal antibody

MTH1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D7G4

Cat no : 67443-1-Ig

Print datasheet

Synonyms

NUDT1, EC:3.6.1.-, 8-oxo-dGTPase, 8 oxo dGTPase, 7,8-dihydro-8-oxoguanine triphosphatase

验证数据展示

  • WB analysis using 67443-1-Ig

    WB analysis using 67443-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67443-1-Ig (MTH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 67443-1-Ig

    IHC staining of human renal cell carcinoma using 67443-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67443-1-Ig (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUDT1/MTH1 antibody included
  • IHC staining of human colon cancer using 67443-1-Ig

    IHC staining of human colon cancer using 67443-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67443-1-Ig (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUDT1/MTH1 antibody included
  • IHC staining of human lung cancer using 67443-1-Ig

    IHC staining of human lung cancer using 67443-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67443-1-Ig (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUDT1/MTH1 antibody included

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman renal cell carcinoma tissue, mouse testis tissue, human colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NUDT1/MTH1 Ready-To-Use IHC Kit. NUDT1/MTH1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67443-1-Ig targets MTH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MTH1 fusion protein Ag9802 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 1
Synonyms NUDT1, EC:3.6.1.-, 8-oxo-dGTPase, 8 oxo dGTPase, 7,8-dihydro-8-oxoguanine triphosphatase
Calculated Molecular Weight179aa,20 kDa; 197aa,23 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC014618
Gene Symbol MTH1
Gene ID (NCBI) 4521
RRIDAB_2882678
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36639
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTH1, also known as NUDT1 or 8-Oxo-dGTPase, is an important repair enzyme that prevents oxidative stress-induced DNA damage through hydrolyzing mutagenic 8-oxo-dGTP into 8-oxo-dGMP. MTH1 is expressed in postmitotic neurons as well as in proliferative tissues, and it is localized both in the mitochondria and nucleus. MTH1 might be a useful marker of oxidative stress and its level increased upon oxidative stress. Increased expression of MTH1 were also found in various cancer tissues and neurodegenerative diseases.

实验方案

Product Specific Protocols
WB protocol for MTH1 antibody 67443-1-IgDownload protocol
IHC protocol for MTH1 antibody 67443-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols