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CoraLite® Plus 488-conjugated MTH1 Monoclonal antibody

MTH1 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D7G4

Cat No : CL488-67443

Print datasheet

Synonyms

8 oxo dGTPase, MTH1, Nudix motif 1, NUDT1

验证数据展示

  • IF Staining of HepG2 using CL488-67443

    IF Staining of HepG2 using CL488-67443

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 MTH1 antibody (CL488-67443, Clone: 2D7G4 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-67443

    FC experiment of Jurkat using CL488-67443

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MTH1 (CL488-67443, Clone:2D7G4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHepG2 cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy NUDT1/MTH1 Ready-To-Use IHC Kit. NUDT1/MTH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67443-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67443 targets MTH1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF/ICC Application Description
Tested Reactivity Human
Immunogen MTH1 fusion protein Ag9802 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 1
Synonyms 8 oxo dGTPase, MTH1, Nudix motif 1, NUDT1
Calculated Molecular Weight179aa,20 kDa; 197aa,23 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC014618
Gene Symbol NUDT1
Gene ID (NCBI) 4521
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP36639
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTH1, also known as NUDT1 or 8-Oxo-dGTPase, is an important repair enzyme that prevents oxidative stress-induced DNA damage through hydrolyzing mutagenic 8-oxo-dGTP into 8-oxo-dGMP. MTH1 is expressed in postmitotic neurons as well as in proliferative tissues, and it is localized both in the mitochondria and nucleus. MTH1 might be a useful marker of oxidative stress and its level increased upon oxidative stress. Increased expression of MTH1 were also found in various cancer tissues and neurodegenerative diseases.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MTH1 antibody CL488-67443Download protocol
FC protocol for CL Plus 488 MTH1 antibody CL488-67443Download protocol
Standard Protocols
Click here to view our Standard Protocols