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验证数据展示

  • IF Staining of HeLa using CL594-67443

    IF Staining of HeLa using CL594-67443

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 MTH1 antibody (CL594-67443, Clone: 2D7G4 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL594-67443

    FC experiment of Jurkat using CL594-67443

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MTH1 (CL594-67443, Clone:2D7G4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated MTH1 Monoclonal antibody

MTH1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67443

产品说明书

CloneNo. 2D7G4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

NUDT1, EC:3.6.1.-, 8-oxo-dGTPase, 8 oxo dGTPase, 7,8-dihydro-8-oxoguanine triphosphatase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy NUDT1/MTH1 Ready-To-Use IHC Kit. NUDT1/MTH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67443-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67443 targets MTH1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 MTH1 fusion protein Ag9802 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nudix (nucleoside diphosphate linked moiety X)-type motif 1
别名 NUDT1, EC:3.6.1.-, 8-oxo-dGTPase, 8 oxo dGTPase, 7,8-dihydro-8-oxoguanine triphosphatase
计算分子量179aa,20 kDa; 197aa,23 kDa
观测分子量 19 kDa
GenBank蛋白编号BC014618
基因名称 MTH1
Gene ID (NCBI) 4521
RRIDAB_3084766
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP36639
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTH1, also known as NUDT1 or 8-Oxo-dGTPase, is an important repair enzyme that prevents oxidative stress-induced DNA damage through hydrolyzing mutagenic 8-oxo-dGTP into 8-oxo-dGMP. MTH1 is expressed in postmitotic neurons as well as in proliferative tissues, and it is localized both in the mitochondria and nucleus. MTH1 might be a useful marker of oxidative stress and its level increased upon oxidative stress. Increased expression of MTH1 were also found in various cancer tissues and neurodegenerative diseases.

实验方案

Product Specific Protocols
IF protocol for CL594 MTH1 antibody CL594-67443Download protocol
Standard Protocols
Click here to view our Standard Protocols