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MTH1 Monoclonal antibody, PBS Only (Capture)

MTH1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D7G4

Cat no : 67443-1-PBS

Print datasheet

Synonyms

NUDT1, EC:3.6.1.-, 8-oxo-dGTPase, 8 oxo dGTPase, 7,8-dihydro-8-oxoguanine triphosphatase

验证数据展示

  • WB analysis using 67443-1-Ig (same clone as 67443-1-PBS)

    WB analysis using 67443-1-Ig (same clone as 67443-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67443-1-Ig (MTH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67443-1-PBS in a different storage buffer formulation.

  • IHC staining of human renal cell carcinoma using 67443-1-Ig (same clone as 67443-1-PBS)

    IHC staining of human renal cell carcinoma using 67443-1-Ig (same clone as 67443-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67443-1-Ig (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67443-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUDT1/MTH1 antibody included
  • IHC staining of human colon cancer using 67443-1-Ig (same clone as 67443-1-PBS)

    IHC staining of human colon cancer using 67443-1-Ig (same clone as 67443-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67443-1-Ig (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67443-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUDT1/MTH1 antibody included
  • IHC staining of human lung cancer using 67443-1-Ig (same clone as 67443-1-PBS)

    IHC staining of human lung cancer using 67443-1-Ig (same clone as 67443-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67443-1-Ig (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67443-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUDT1/MTH1 antibody included
  • Cytometric bead array standard curve of MP50783-2

    Cytometric bead array standard curve of MP50783-2

    Cytometric bead array standard curve of MP50783-2, NUDT1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67443-1-PBS. Detection antibody: 67443-4-PBS. Standard:Ag9802. Range: 0.195-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy NUDT1/MTH1 Ready-To-Use IHC Kit. NUDT1/MTH1 primary antibody included.

产品信息

67443-1-PBS targets MTH1 as part of a matched antibody pair:

MP50783-2: 67443-1-PBS capture and 67443-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen MTH1 fusion protein Ag9802 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 1
Synonyms NUDT1, EC:3.6.1.-, 8-oxo-dGTPase, 8 oxo dGTPase, 7,8-dihydro-8-oxoguanine triphosphatase
Calculated Molecular Weight179aa,20 kDa; 197aa,23 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC014618
Gene Symbol MTH1
Gene ID (NCBI) 4521
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36639
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MTH1, also known as NUDT1 or 8-Oxo-dGTPase, is an important repair enzyme that prevents oxidative stress-induced DNA damage through hydrolyzing mutagenic 8-oxo-dGTP into 8-oxo-dGMP. MTH1 is expressed in postmitotic neurons as well as in proliferative tissues, and it is localized both in the mitochondria and nucleus. MTH1 might be a useful marker of oxidative stress and its level increased upon oxidative stress. Increased expression of MTH1 were also found in various cancer tissues and neurodegenerative diseases.