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MCM7 Monoclonal antibody

MCM7 Monoclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G1F6

Cat no : 67446-1-Ig

Print datasheet

Synonyms

CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146

验证数据展示

  • WB analysis using 67446-1-Ig

    WB analysis using 67446-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67446-1-Ig (MCM7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67446-1-Ig

    IHC staining of human breast cancer using 67446-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCM7 antibody included
  • IP experiment of HeLa using 67446-1-Ig

    IP experiment of HeLa using 67446-1-Ig

    IP result of anti-MCM7 (IP:67446-1-Ig, 4ug; Detection:67446-1-Ig 1:2000) with HeLa cells lysate 1040 ug.

经过测试的应用

Positive WB detected inA431 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MCM7 Ready-To-Use IHC Kit. MCM7 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67446-1-Ig targets MCM7 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen MCM7 fusion protein Ag28558 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name minichromosome maintenance complex component 7
Synonyms CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC013375
Gene Symbol MCM7
Gene ID (NCBI) 4176
RRIDAB_2882680
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP33993
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2–7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].

实验方案

Product Specific Protocols
WB protocol for MCM7 antibody 67446-1-IgDownload protocol
IHC protocol for MCM7 antibody 67446-1-IgDownload protocol
IP protocol for MCM7 antibody 67446-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols