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验证数据展示

  • IF Staining of HepG2 using CL488-67446

    IF Staining of HepG2 using CL488-67446

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MCM7 antibody (CL488-67446, Clone: 1G1F6 ) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated MCM7 Monoclonal antibody

MCM7 Monoclonal Antibody for IF/ICC
Cat No. CL488-67446

产品说明书

CloneNo. 1G1F6

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

IF/ICC

别名

CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MCM7 Ready-To-Use IHC Kit. MCM7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67446-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67446 targets MCM7 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat
免疫原 MCM7 fusion protein Ag28558 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 minichromosome maintenance complex component 7
别名 CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146
计算分子量 81 kDa
观测分子量 85 kDa
GenBank蛋白编号BC013375
基因名称 MCM7
Gene ID (NCBI) 4176
RRIDAB_2919474
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP33993
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2-7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MCM7 antibody CL488-67446Download protocol
Standard Protocols
Click here to view our Standard Protocols