验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67446-1-Ig targets MCM7 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
经测试反应性 | Human, mouse, rat |
免疫原 | MCM7 fusion protein Ag28558 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | minichromosome maintenance complex component 7 |
别名 | CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146 |
计算分子量 | 81 kDa |
观测分子量 | 85 kDa |
GenBank蛋白编号 | BC013375 |
基因名称 | MCM7 |
Gene ID (NCBI) | 4176 |
RRID | AB_2882680 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P33993 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2–7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].
实验方案
Product Specific Protocols | |
---|---|
WB protocol for MCM7 antibody 67446-1-Ig | Download protocol |
IHC protocol for MCM7 antibody 67446-1-Ig | Download protocol |
IP protocol for MCM7 antibody 67446-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |