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Host
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验证数据展示

  • WB analysis using 67446-1-Ig (same clone as 67446-1-PBS)

    WB analysis using 67446-1-Ig (same clone as 67446-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67446-1-Ig (MCM7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67446-1-Ig (same clone as 67446-1-PBS)

    IHC staining of human breast cancer using 67446-1-Ig (same clone as 67446-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCM7 antibody included
  • IP experiment of HeLa using 67446-1-Ig (same clone as 67446-1-PBS)

    IP experiment of HeLa using 67446-1-Ig (same clone as 67446-1-PBS)

    IP result of anti-MCM7 (IP:67446-1-Ig, 4ug; Detection:67446-1-Ig 1:2000) with HeLa cells lysate 1040 ug. This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.

MCM7 Monoclonal antibody, PBS Only

MCM7 Monoclonal Antibody for WB, IP, IHC, Indirect ELISA
Cat No. 67446-1-PBS

产品说明书

CloneNo. 1G1F6

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IP, IHC, Indirect ELISA

别名

CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MCM7 Ready-To-Use IHC Kit. MCM7 primary antibody included.

产品信息

67446-1-PBS targets MCM7 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IP, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 MCM7 fusion protein Ag28558 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 minichromosome maintenance complex component 7
别名 CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146
计算分子量 81 kDa
观测分子量 85 kDa
GenBank蛋白编号BC013375
基因名称 MCM7
Gene ID (NCBI) 4176
RRIDAB_2882680
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP33993
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2–7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].