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MCM7 Monoclonal antibody, PBS Only

MCM7 Monoclonal Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1F6

Cat no : 67446-1-PBS

Print datasheet

Synonyms

CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146

验证数据展示

  • WB analysis using 67446-1-Ig (same clone as 67446-1-PBS)

    WB analysis using 67446-1-Ig (same clone as 67446-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67446-1-Ig (MCM7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67446-1-Ig (same clone as 67446-1-PBS)

    IHC staining of human breast cancer using 67446-1-Ig (same clone as 67446-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCM7 antibody included
  • IP experiment of HeLa using 67446-1-Ig (same clone as 67446-1-PBS)

    IP experiment of HeLa using 67446-1-Ig (same clone as 67446-1-PBS)

    IP result of anti-MCM7 (IP:67446-1-Ig, 4ug; Detection:67446-1-Ig 1:2000) with HeLa cells lysate 1040 ug. This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MCM7 Ready-To-Use IHC Kit. MCM7 primary antibody included.

产品信息

67446-1-PBS targets MCM7 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IP, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen MCM7 fusion protein Ag28558 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name minichromosome maintenance complex component 7
Synonyms CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146
Calculated Molecular Weight 81 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC013375
Gene Symbol MCM7
Gene ID (NCBI) 4176
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP33993
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2–7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].