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OMA1 Monoclonal antibody, PBS Only (Detector)

OMA1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H4D12

Cat no : 67449-1-PBS

Print datasheet

Synonyms

Overlapping with the m-AAA protease 1 homolog, MPRP-1, MPRP1, Metalloprotease-related protein 1, EC:3.4.24.-

验证数据展示

  • WB analysis of NIH/3T3 using 67449-1-Ig (same clone as 67449-1-PBS)

    WB analysis of NIH/3T3 using 67449-1-Ig (same clone as 67449-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67449-1-PBS in a different storage buffer formulation.

  • WB analysis using 67449-1-Ig (same clone as 67449-1-PBS)

    WB analysis using 67449-1-Ig (same clone as 67449-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67449-1-Ig (OMA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67449-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50571-3

    Cytometric bead array standard curve of MP50571-3

    Cytometric bead array standard curve of MP50571-3, OMA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66572-1-PBS. Detection antibody: 67449-1-PBS. Standard:Ag11201. Range: 3.125-100 ng/mL.

产品信息

67449-1-PBS targets OMA1 as part of a matched antibody pair:

MP50571-3: 66572-1-PBS capture and 67449-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen OMA1 fusion protein Ag11201 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name OMA1 homolog, zinc metallopeptidase (S. cerevisiae)
Synonyms Overlapping with the m-AAA protease 1 homolog, MPRP-1, MPRP1, Metalloprotease-related protein 1, EC:3.4.24.-
Calculated Molecular Weight 524 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC012915
Gene Symbol OMA1
Gene ID (NCBI) 115209
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96E52
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OMA1(Overlapping with the m-AAA protease 1 homolog) is also named as MPRP1(Metalloprotease-related protein 1) and belongs to the peptidase M48 family.It is a zinc metalloprotease that spans the inner mitochondrial membrane with a number of predicted membrane spanning domains and the 60 kDa form of OMA1 rapidly accumulated concomitantly with the cleavage of all OPA1 variants,and the 40 kDa form of OMA1 may be the inactive form, with the 60 kDa form being the active enzyme(PMID:20334834)