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  • Featured Product
  • KD/KO Validated

TAOK3 Monoclonal antibody

TAOK3 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1A5A9

Cat no : 67451-1-Ig

Print datasheet

Synonyms

DKFZp666H245, DPK, FLJ31808, hKFC A, JIK, JNK/SAPK inhibitory kinase, Jun kinase inhibitory kinase, KDS, Kinase from chicken homolog A, MAP3K18, TAO kinase 3, TAOK3

验证数据展示

  • WB analysis using 67451-1-Ig

    WB analysis using 67451-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67451-1-Ig (TAOK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 67451-1-Ig

    IHC staining of human prostate cancer using 67451-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67451-1-Ig (TAOK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 67451-1-Ig

    IHC staining of human prostate cancer using 67451-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67451-1-Ig (TAOK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human prostate cancer using 67451-1-Ig

    IF Staining of human prostate cancer using 67451-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using TAOK3 antibody (67451-1-Ig, Clone: 1A5A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human prostate cancer using 67451-1-Ig

    IF Staining of human prostate cancer using 67451-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using TAOK3 antibody (67451-1-Ig, Clone: 1A5A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of mouse kidney using 67451-1-Ig

    IHC staining of mouse kidney using 67451-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67451-1-Ig (TAOK3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, A549 cells, LNCaP cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67451-1-Ig targets TAOK3 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen TAOK3 fusion protein Ag29146 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TAO kinase 3
Synonyms DKFZp666H245, DPK, FLJ31808, hKFC A, JIK, JNK/SAPK inhibitory kinase, Jun kinase inhibitory kinase, KDS, Kinase from chicken homolog A, MAP3K18, TAO kinase 3, TAOK3
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 100-105 kDa
GenBank Accession NumberBC002756
Gene Symbol TAOK3
Gene ID (NCBI) 51347
RRIDAB_2882685
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H2K8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAOK3(Serine/threonine-protein kinase TAO3) is also named as DPK, JIK, KDS, MAP3K18 and belongs to the STE Ser/Thr protein kinase family. It acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, it is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. And this protein can be autophosphorylated(PMID:20949042).

实验方案

Product Specific Protocols
WB protocol for TAOK3 antibody 67451-1-IgDownload protocol
IHC protocol for TAOK3 antibody 67451-1-IgDownload protocol
IF protocol for TAOK3 antibody 67451-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,CoIP

Adv Sci (Weinh)

TAOK3 Facilitates Esophageal Squamous Cell Carcinoma Progression and Cisplatin Resistance Through Augmenting Autophagy Mediated by IRGM

Authors - Mingchuang Sun
  • KD Validated
View Article