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Beta Arrestin 1 Monoclonal antibody

Beta Arrestin 1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H8E4

Cat no : 67580-1-Ig

Print datasheet

Synonyms

ARB1, ARR1, ARRB1, Arrestin beta 1, arrestin, beta 1, Beta arrestin 1

验证数据展示

  • WB analysis using 67580-1-Ig

    WB analysis using 67580-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67580-1-Ig (Arrestin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67580-1-Ig

    WB analysis of HepG2 using 67580-1-Ig

    WB result of Beta Arrestin 1 antibody (67580-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Arrestin 1 transfected HepG2 cells.

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, NIH/3T3 cells, human peripheral blood platelets, K-562 cells, Jurkat cells, HSC-T6 cells
Planning an IHC experiment? We recommend our IHCeasy ARRB1 Ready-To-Use IHC Kit. ARRB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67580-1-Ig targets Beta Arrestin 1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Beta Arrestin 1 fusion protein Ag7504 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name arrestin, beta 1
Synonyms ARB1, ARR1, ARRB1, Arrestin beta 1, arrestin, beta 1, Beta arrestin 1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 45-55 kDa
GenBank Accession NumberBC003636
Gene Symbol Beta Arrestin 1
Gene ID (NCBI) 408
RRIDAB_2882791
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49407
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).

实验方案

Product Specific Protocols
WB protocol for Beta Arrestin 1 antibody 67580-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols