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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Beta Arrestin 1 Monoclonal antibody

Beta Arrestin 1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H8E4

Cat no : CL488-67580

Print datasheet

Synonyms

ARRB1, ARR1, ARR 1, ARB2, ARB1

验证数据展示

  • IF Staining of MCF-7 using CL488-67580

    IF Staining of MCF-7 using CL488-67580

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 Beta Arrestin 1 antibody (CL488-67580, Clone: 1H8E4 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67580

    FC experiment of HeLa using CL488-67580

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Beta Arrestin 1 (CL488-67580, Clone:1H8E4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARRB1 Ready-To-Use IHC Kit. ARRB1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67580-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67580 targets Beta Arrestin 1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Beta Arrestin 1 fusion protein Ag7504 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name arrestin, beta 1
Synonyms ARRB1, ARR1, ARR 1, ARB2, ARB1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC003636
Gene Symbol Beta Arrestin 1
Gene ID (NCBI) 408
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49407
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of beta Arrestin 1 in saliva has been identified as a candidate circadian biomarker.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Beta Arrestin 1 antibody CL488-67580Download protocol
FC protocol for CL Plus 488 Beta Arrestin 1 antibody CL488-67580Download protocol
Standard Protocols
Click here to view our Standard Protocols