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验证数据展示

  • WB analysis using 67580-1-Ig

    WB analysis using 67580-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67580-1-Ig (Arrestin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67580-1-Ig

    IHC staining of mouse brain using 67580-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67580-1-Ig (Beta Arrestin 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARRB1 antibody included
  • WB analysis of HepG2 using 67580-1-Ig

    WB analysis of HepG2 using 67580-1-Ig

    WB result of Beta Arrestin 1 antibody (67580-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Arrestin 1 transfected HepG2 cells.

  • Featured Product
  • KD/KO Validated

Beta Arrestin 1 Monoclonal antibody

Beta Arrestin 1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67580-1-Ig

Print datasheet

CloneNo. 1H8E4

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ARRB1, ARR1, ARR 1, ARB2, ARB1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, NIH/3T3 cells, human peripheral blood platelets, K-562 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ARRB1 Ready-To-Use IHC Kit. ARRB1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67580-1-Ig targets Beta Arrestin 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Beta Arrestin 1 fusion protein Ag7504 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name arrestin, beta 1
Synonyms ARRB1, ARR1, ARR 1, ARB2, ARB1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 45-55 kDa
GenBank Accession NumberBC003636
Gene Symbol Beta Arrestin 1
Gene ID (NCBI) 408
RRIDAB_2882791
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49407
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).

实验方案

Product Specific Protocols
WB protocol for Beta Arrestin 1 antibody 67580-1-IgDownload protocol
IHC protocol for Beta Arrestin 1 antibody 67580-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols