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验证数据展示

  • WB analysis using 67652-1-Ig

    WB analysis using 67652-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67652-1-Ig (TCF7L1/TCF7L2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

TCF7L1/TCF7L2 Monoclonal antibody

TCF7L1/TCF7L2 Monoclonal Antibody for WB, ELISA
Cat No. 67652-1-Ig

产品说明书

CloneNo. 2B2D12

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse

应用

WB, ELISA

别名

HMG box transcription factor 3, HMG box transcription factor 4, TCF 3, TCF 4, TCF3, TCF4, TCF7L1, TCF7L2, Transcription factor 7 like 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, A431 cells, NCCIT cells, JAR cells, NIH/3T3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67652-1-Ig targets TCF7L1/TCF7L2 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 TCF7L1/TCF7L2 fusion protein Ag28954 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 transcription factor 7-like 1 (T-cell specific, HMG-box)
别名 HMG box transcription factor 3, HMG box transcription factor 4, TCF 3, TCF 4, TCF3, TCF4, TCF7L1, TCF7L2, Transcription factor 7 like 1
计算分子量 63 kDa
观测分子量 70 kDa
GenBank蛋白编号BC058894
基因名称 TCF7L1
Gene ID (NCBI) 83439
RRIDAB_2882851
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9HCS4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis. This antibody raised against 1-200aa of human TCF7L1 can also react with TCF7L2.

实验方案

Product Specific Protocols
WB protocol for TCF7L1/TCF7L2 antibody 67652-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols