验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, human platelet, pig rectum, rat rectum, pig brain, rat brain, mouse brain |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67657-1-Ig targets Destrin in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse, rat, pig |
| 文献引用反应性 | human |
| 免疫原 | Destrin fusion protein Ag1402 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | destrin (actin depolymerizing factor) |
| 别名 | ACTDP, Actin depolymerizing factor, ADF, bA462D18.2, Destrin, DSN, DSTN |
| 计算分子量 | 19 kDa |
| 观测分子量 | 18-19 kDa |
| GenBank蛋白编号 | BC009477 |
| 基因名称 | Destrin |
| Gene ID (NCBI) | 11034 |
| RRID | AB_2918496 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P60981 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Destrin (also known as actin depolymerization factor, ADF) is an actin-binding protein contributing to cytoskeletal dynamics by promoting rapid actin filament disassembly. By regulating actin cytoskeletal reorganization it plays an important role in the formation of filopodia, which further promotes tumor cell invasion and metastasis. Destrin has been reported to be overexpressed in primary colon cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Destrin antibody 67657-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |