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ETFA Monoclonal antibody

ETFA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C12G5

Cat no : 67661-1-Ig

Print datasheet

Synonyms

Alpha ETF, EMA, ETFA, GA2, MADD

验证数据展示

  • WB analysis of mouse brain using 67661-1-Ig

    WB analysis of mouse brain using 67661-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67661-1-Ig

    WB analysis using 67661-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67661-1-Ig

    IF Staining of HepG2 using 67661-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ETFA antibody (67661-1-Ig, Clone: 3C12G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

  • IHC staining of human liver cancer using 67661-1-Ig

    IHC staining of human liver cancer using 67661-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67661-1-Ig (ETFA antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ETFA antibody included
  • IHC staining of human liver cancer using 67661-1-Ig

    IHC staining of human liver cancer using 67661-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67661-1-Ig (ETFA antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ETFA antibody included
  • WB analysis using 67661-1-Ig

    WB analysis using 67661-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHCT 116 cells, LNCaP cells, mouse brain tissue, HuH-7 cells, HeLa cells, K-562 cells, pig liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ETFA Ready-To-Use IHC Kit. ETFA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:8000-1:32000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67661-1-Ig targets ETFA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen ETFA fusion protein Ag30257 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name electron-transfer-flavoprotein, alpha polypeptide
Synonyms Alpha ETF, EMA, ETFA, GA2, MADD
Calculated Molecular Weight 30 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC015526
Gene Symbol ETFA
Gene ID (NCBI) 2108
RRIDAB_2882858
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13804
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The electron transfer flavoprotein(ETF), located in the mitochondrial matrix, is a nuclear-encoded enzyme delivering to the respiratory chain by straight-chain acyl-CoA dehydrogenases and other dehydrogenases. Mitochondrial fatty acids oxidation and amino acids metabolism are affected in MADD(Multiple acyl-CoA dehydrogenase deficiency). These disorders are linked to a dysfunction of ETF. ETF is a mitochondrial matrix protein consisting of alpha- (30kDa) and beta- (28kDa) subunits encoded by the ETFA and ETFB genes, respectively. (PMID: 31996215,PMID: 16510302)

实验方案

Product Specific Protocols
WB protocol for ETFA antibody 67661-1-IgDownload protocol
IHC protocol for ETFA antibody 67661-1-IgDownload protocol
IF protocol for ETFA antibody 67661-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols