IHCeasy ETFA Ready-To-Use IHC Kit
ETFA Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse, Rat
Sample Type
FFPE tissue
Cat No : KHC0544
Synonyms
Alpha ETF, EMA, ETFA, GA2, MADD
Validation Data Gallery
Product Information
KHC0544 is a ready-to-use IHC kit for staining of ETFA. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy ETFA Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Mouse Monoclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Mouse |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
The electron transfer flavoprotein(ETF), located in the mitochondrial matrix, is a nuclear-encoded enzyme delivering to the respiratory chain by straight-chain acyl-CoA dehydrogenases and other dehydrogenases. Mitochondrial fatty acids oxidation and amino acids metabolism are affected in MADD(Multiple acyl-CoA dehydrogenase deficiency). These disorders are linked to a dysfunction of ETF. ETF is a mitochondrial matrix protein consisting of alpha- and beta- subunits encoded by the ETFA and ETFB genes, respectively.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | Alpha ETF, EMA, ETFA, GA2, MADD |
