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ETFA Monoclonal antibody, PBS Only

ETFA Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C12G5

Cat no : 67661-1-PBS

Print datasheet

Synonyms

Alpha ETF, EMA, ETFA, GA2, MADD

验证数据展示

  • WB analysis of mouse brain using 67661-1-Ig (same clone as 67661-1-PBS)

    WB analysis of mouse brain using 67661-1-Ig (same clone as 67661-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

  • WB analysis using 67661-1-Ig (same clone as 67661-1-PBS)

    WB analysis using 67661-1-Ig (same clone as 67661-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67661-1-Ig (same clone as 67661-1-PBS)

    IF Staining of HepG2 using 67661-1-Ig (same clone as 67661-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ETFA antibody (67661-1-Ig, Clone: 3C12G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (blue). This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67661-1-Ig (same clone as 67661-1-PBS)

    IHC staining of human liver cancer using 67661-1-Ig (same clone as 67661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67661-1-Ig (ETFA antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ETFA antibody included
  • IHC staining of human liver cancer using 67661-1-Ig (same clone as 67661-1-PBS)

    IHC staining of human liver cancer using 67661-1-Ig (same clone as 67661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67661-1-Ig (ETFA antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ETFA antibody included
  • WB analysis using 67661-1-Ig (same clone as 67661-1-PBS)

    WB analysis using 67661-1-Ig (same clone as 67661-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67661-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ETFA Ready-To-Use IHC Kit. ETFA primary antibody included.

产品信息

67661-1-PBS targets ETFA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen ETFA fusion protein Ag30257 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name electron-transfer-flavoprotein, alpha polypeptide
Synonyms Alpha ETF, EMA, ETFA, GA2, MADD
Calculated Molecular Weight 30 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC015526
Gene Symbol ETFA
Gene ID (NCBI) 2108
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13804
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The electron transfer flavoprotein(ETF), located in the mitochondrial matrix, is a nuclear-encoded enzyme delivering to the respiratory chain by straight-chain acyl-CoA dehydrogenases and other dehydrogenases. Mitochondrial fatty acids oxidation and amino acids metabolism are affected in MADD(Multiple acyl-CoA dehydrogenase deficiency). These disorders are linked to a dysfunction of ETF. ETF is a mitochondrial matrix protein consisting of alpha- (30kDa) and beta- (28kDa) subunits encoded by the ETFA and ETFB genes, respectively. (PMID: 31996215,PMID: 16510302)