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验证数据展示

  • IHC staining of mouse pancreas using 67668-1-Ig

    IHC staining of mouse pancreas using 67668-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67668-1-Ig (Ins1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 67668-1-Ig

    IHC staining of mouse pancreas using 67668-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67668-1-Ig (Ins1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse pancreas using 67668-1-Ig

    IF Staining of mouse pancreas using 67668-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Ins1 antibody (67668-1-Ig, Clone: 1H2C11 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Ins1 Monoclonal antibody

Ins1 Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 67668-1-Ig

产品说明书

CloneNo. 1H2C11

宿主/亚型

Mouse / IgG1

种属反应性

mouse, rat

应用

IHC, IF-P, ELISA

别名

Insulin, insulin1, insulin I, Ins2 rs1, Ins-1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse pancreas tissue, rat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67668-1-Ig targets Ins1 in IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Ins1 fusion protein Ag29988 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 insulin I
别名 Insulin, insulin1, insulin I, Ins2 rs1, Ins-1
计算分子量 12 kDa
GenBank蛋白编号NM_008386
基因名称 Ins1
Gene ID (NCBI) 16333
RRIDAB_2882864
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01325
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

insulin I is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.

实验方案

Product Specific Protocols
IHC protocol for Ins1 antibody 67668-1-IgDownload protocol
IF protocol for Ins1 antibody 67668-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols