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验证数据展示

  • IF Staining of mouse pancreas using CL594-67668

    IF Staining of mouse pancreas using CL594-67668

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite®594-conjugated Ins1 antibody (CL594-67668, Clone: 1H2C11 ) at dilution of 1:300.

CoraLite®594-conjugated Ins1 Monoclonal antibody

Ins1 Monoclonal Antibody for IF-P
Cat No. CL594-67668

Print datasheet

CloneNo. 1H2C11

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat

Applications

IF-P

Synonyms

Ins 1, Ins1, Ins2 rs1, insulin I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse pancreas tissue
For other applications, we recommend the unconjugated version of this antibody, 67668-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67668 targets Ins1 in IF-P applications and shows reactivity with mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity mouse, rat
Immunogen Ins1 fusion protein Ag29988 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name insulin I
Synonyms Ins 1, Ins1, Ins2 rs1, insulin I
Calculated Molecular Weight 12 kDa
GenBank Accession NumberNM_008386
Gene Symbol Ins1
Gene ID (NCBI) 16333
RRIDAB_2920169
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP01325
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

insulin I is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.

实验方案

Product Specific Protocols
IF protocol for CL594 Ins1 antibody CL594-67668Download protocol
Standard Protocols
Click here to view our Standard Protocols