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Ins1 Monoclonal antibody

Ins1 Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1H2C11

Cat no : 67668-1-Ig

Print datasheet

Synonyms

Insulin, insulin1, insulin I, Ins2 rs1, Ins-1

验证数据展示

  • IHC staining of mouse pancreas using 67668-1-Ig

    IHC staining of mouse pancreas using 67668-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67668-1-Ig (Ins1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 67668-1-Ig

    IHC staining of mouse pancreas using 67668-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67668-1-Ig (Ins1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse pancreas using 67668-1-Ig

    IF Staining of mouse pancreas using 67668-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Ins1 antibody (67668-1-Ig, Clone: 1H2C11 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive IHC detected inmouse pancreas tissue, rat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67668-1-Ig targets Ins1 in IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Ins1 fusion protein Ag29988 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name insulin I
Synonyms Insulin, insulin1, insulin I, Ins2 rs1, Ins-1
Calculated Molecular Weight 12 kDa
GenBank Accession NumberNM_008386
Gene Symbol Ins1
Gene ID (NCBI) 16333
RRIDAB_2882864
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01325
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

insulin I is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.

实验方案

Product Specific Protocols
IHC protocol for Ins1 antibody 67668-1-IgDownload protocol
IF protocol for Ins1 antibody 67668-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols