验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human lung cancer tissue, human urothelial carcinoma tissue, human colon cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive FC (Intra) detected in | HEK-293 cells |
This antibody is not recommended for immunocytofluorescent assays.
Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67733-1-Ig targets METTL3 in WB, IHC, IF-P, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | METTL3 fusion protein Ag7110 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | methyltransferase like 3 |
| 别名 | MTA70, MT A70, Methyltransferase-like protein 3, hMETTL3, EC:2.1.1.348 |
| 计算分子量 | 64 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC001650 |
| 基因名称 | METTL3 |
| Gene ID (NCBI) | 56339 |
| RRID | AB_2918502 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q86U44 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for METTL3 antibody 67733-1-Ig | Download protocol |
| IHC protocol for METTL3 antibody 67733-1-Ig | Download protocol |
| IF protocol for METTL3 antibody 67733-1-Ig | Download protocol |
| FC protocol for METTL3 antibody 67733-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Environ Pollut RNA reading protein YTHDF2 mediates Benzo(k)fluoranthene induced male reproductive injury by regulating the stability of BCL2 | ||
EMBO Rep DDX5 inhibits inflammation by modulating m6A levels of TLR2/4 transcripts during bacterial infection | ||
Cancer Lett Therapeutic targeting m6A-guided miR-146a-5p signaling contributes to the melittin-induced selective suppression of bladder cancer. | ||
J Invest Dermatol NPTX2 Promotes EMT in Cutaneous Squamous Cell Carcinoma via Mettl3-Mediated m6A Methylation of Snail | ||
Am J Respir Cell Mol Biol The Combined Effects of Circular RNA Methylation Promote Pulmonary Fibrosis.
| ||
Transl Oncol METTL3 methylated KIF15 promotes nasopharyngeal carcinoma progression and radiation resistance by blocking ATG7-mediated autophagy through the activation of STAT3 pathway |
