验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse testis tissue |
| Positive FC (Intra) detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67733-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67733 targets METTL3 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF-P, FC (Intra) Application Description |
| 文献引用应用 | FC (Intra) |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | METTL3 fusion protein Ag7110 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | methyltransferase like 3 |
| 别名 | MTA70, MT A70, Methyltransferase-like protein 3, hMETTL3, EC:2.1.1.348 |
| 计算分子量 | 64 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC001650 |
| 基因名称 | METTL3 |
| Gene ID (NCBI) | 56339 |
| RRID | AB_2920176 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q86U44 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 METTL3 antibody CL594-67733 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
