验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
HRP-67733 targets METTL3 in WB applications and shows reactivity with Human, mouse samples.
经测试应用 | WB Application Description |
经测试反应性 | Human, mouse |
免疫原 | METTL3 fusion protein Ag7110 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | methyltransferase like 3 |
别名 | IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8 |
计算分子量 | 64 kDa |
观测分子量 | 65-70 kDa |
GenBank蛋白编号 | BC001650 |
基因名称 | METTL3 |
Gene ID (NCBI) | 56339 |
RRID | AB_2920513 |
偶联类型 | HRP |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q86U44 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for HRP METTL3 antibody HRP-67733 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |