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验证数据展示

  • WB analysis of HeLa using HRP-67733

    WB analysis of HeLa using HRP-67733

    HeLa cells were subjected to SDS PAGE followed by western blot with HRP-67733 (METTL3 antibody) at dilutions of 1:5000, 1:10000 and 1:20000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

HRP-conjugated METTL3 Monoclonal antibody

METTL3 Monoclonal Antibody for WB
Cat No. HRP-67733

Print datasheet

CloneNo. 2D8H1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB

Synonyms

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  HRP
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy METTL3 Ready-To-Use IHC Kit. METTL3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-67733 targets METTL3 in WB applications and shows reactivity with Human, mouse samples.

Tested Applications WB Application Description
Tested Reactivity Human, mouse
Immunogen METTL3 fusion protein Ag7110 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name methyltransferase like 3
Synonyms IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8
Calculated Molecular Weight 64 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC001650
Gene Symbol METTL3
Gene ID (NCBI) 56339
RRIDAB_2920513
Conjugate HRP
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86U44
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

实验方案

Product Specific Protocols
WB protocol for HRP METTL3 antibody HRP-67733Download protocol
Standard Protocols
Click here to view our Standard Protocols