验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, A431 cells, rat brain, mouse brain, chicken brain, HEK-293 cells, HepG2 cells |
| Positive IHC detected in | mouse heart tissue, rat brain tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy GOT2 Ready-To-Use IHC Kit. GOT2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67738-1-Ig targets GOT2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, chicken samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Tested Reactivity | Human, mouse, rat, pig, chicken |
| Immunogen | GOT2 fusion protein Ag6600 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) |
| Synonyms | FABP 1, FABPpm, Fatty acid binding protein, FLJ40994, GOT2, KAT4, KATIV, mAspAT, mitAAT, Transaminase A |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 39-40 kDa |
| GenBank Accession Number | BC000525 |
| Gene Symbol | GOT2 |
| Gene ID (NCBI) | 2806 |
| RRID | AB_2918507 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P00505 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GOT2 antibody 67738-1-Ig | Download protocol |
| IHC protocol for GOT2 antibody 67738-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
