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验证数据展示

  • FC experiment of HeLa using CL488-67738

    FC experiment of HeLa using CL488-67738

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GOT2 (CL488-67738, Clone:1B6C6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated GOT2 Monoclonal antibody

GOT2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67738

Print datasheet

CloneNo. 1B6C6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, chicken

Applications

FC (Intra)

Synonyms

FABP-1, FABP 1, EC:2.6.1.1, Aspartate aminotransferase, mitochondrial, AATM

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GOT2 Ready-To-Use IHC Kit. GOT2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67738-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67738 targets GOT2 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig, chicken samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig, chicken
Immunogen GOT2 fusion protein Ag6600 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
Synonyms FABP-1, FABP 1, EC:2.6.1.1, Aspartate aminotransferase, mitochondrial, AATM
Calculated Molecular Weight 47 kDa
Observed Molecular Weight39-40 kDa
GenBank Accession NumberBC000525
Gene Symbol GOT2
Gene ID (NCBI) 2806
RRIDAB_2923785
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP00505
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 GOT2 antibody CL488-67738Download protocol
Standard Protocols
Click here to view our Standard Protocols