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验证数据展示

  • FC experiment of HeLa using CL488-67738

    FC experiment of HeLa using CL488-67738

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GOT2 (CL488-67738, Clone:1B6C6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated GOT2 Monoclonal antibody

GOT2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67738

产品说明书

CloneNo. 1B6C6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, chicken

应用

FC (Intra)

别名

FABP-1, FABP 1, EC:2.6.1.1, Aspartate aminotransferase, mitochondrial, AATM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GOT2 Ready-To-Use IHC Kit. GOT2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67738-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67738 targets GOT2 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig, chicken samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig, chicken
免疫原 GOT2 fusion protein Ag6600 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
别名 FABP-1, FABP 1, EC:2.6.1.1, Aspartate aminotransferase, mitochondrial, AATM
计算分子量 47 kDa
观测分子量39-40 kDa
GenBank蛋白编号BC000525
基因名称 GOT2
Gene ID (NCBI) 2806
RRIDAB_2923785
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP00505
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 GOT2 antibody CL488-67738Download protocol
Standard Protocols
Click here to view our Standard Protocols