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验证数据展示

  • WB analysis using 67738-1-Ig

    WB analysis using 67738-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67738-1-Ig (GOT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 67738-1-Ig

    IHC staining of mouse heart using 67738-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GOT2 antibody included
  • IHC staining of rat heart using 67738-1-Ig

    IHC staining of rat heart using 67738-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GOT2 antibody included
  • IHC staining of rat brain using 67738-1-Ig

    IHC staining of rat brain using 67738-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GOT2 antibody included
  • IHC staining of rat heart using 67738-1-Ig

    IHC staining of rat heart using 67738-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GOT2 antibody included

GOT2 Monoclonal antibody

GOT2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67738-1-Ig

产品说明书

CloneNo. 1B6C6

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig, chicken

应用

WB, IHC, ELISA

别名

FABP 1, FABPpm, Fatty acid binding protein, FLJ40994, GOT2, KAT4, KATIV, mAspAT, mitAAT, Transaminase A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig brain tissue, A431 cells, rat brain, mouse brain, chicken brain, HEK-293 cells, HepG2 cells
Positive IHC detected inmouse heart tissue, rat brain tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GOT2 Ready-To-Use IHC Kit. GOT2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67738-1-Ig targets GOT2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, chicken samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig, chicken
免疫原 GOT2 fusion protein Ag6600 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
别名 FABP 1, FABPpm, Fatty acid binding protein, FLJ40994, GOT2, KAT4, KATIV, mAspAT, mitAAT, Transaminase A
计算分子量 47 kDa
观测分子量39-40 kDa
GenBank蛋白编号BC000525
基因名称 GOT2
Gene ID (NCBI) 2806
RRIDAB_2918507
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP00505
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.

实验方案

Product Specific Protocols
WB protocol for GOT2 antibody 67738-1-IgDownload protocol
IHC protocol for GOT2 antibody 67738-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols