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验证数据展示

  • WB analysis using 67804-1-Ig

    WB analysis using 67804-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67804-1-Ig (WWP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • WB analysis of HaCaT using 67804-1-Ig

    WB analysis of HaCaT using 67804-1-Ig

    HaCaT cells were subjected to SDS PAGE followed by western blot with 67804-1-Ig (WWP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

WWP1 Monoclonal antibody

WWP1 Monoclonal Antibody for WB, ELISA
Cat No. 67804-1-Ig

产品说明书

CloneNo. 2G8G11

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, ELISA

别名

AIP5, DKFZp434D2111, hSDRP1, Tiul1, WW domain containing protein 1, WWP1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HaCaT cells, HeLa cells, LNCaP cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy WWP1 Ready-To-Use IHC Kit. WWP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67804-1-Ig targets WWP1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 WWP1 fusion protein Ag30171 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 WW domain containing E3 ubiquitin protein ligase 1
别名 AIP5, DKFZp434D2111, hSDRP1, Tiul1, WW domain containing protein 1, WWP1
计算分子量 922 aa, 105 kDa
观测分子量 95-105 kDa
GenBank蛋白编号BC036065
基因名称 WWP1
Gene ID (NCBI) 11059
RRIDAB_2918567
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H0M0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for WWP1 antibody 67804-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols